Botulinum toxin type A products such as Botulax are widely used in aesthetic medicine for reducing dynamic wrinkles and improving facial contours. In its original form, Botulax comes as a vacuum-sealed lyophilized powder. This form ensures stability and long shelf life.
However, before injection, Botulax must be properly prepared with sterile saline through a process called reconstitution or “mixing” to activate the solution and make it suitable for clinical use. Proper handling ensures stability and maintains the biological activity of the toxin.
How to Mix Botulax 100? (Step-by-Step Guide)
Step 1: Prepare sterile equipment
Use sterile gloves, syringes, and alcohol wipes. Ensure all materials are clean to maintain aseptic conditions.
Step 2: Use the correct diluent
The most commonly used solution is 0.9% sterile saline. No preservatives should be added unless specified by clinical protocol.
Some practitioners use 2.5 mL of sterile saline for Botulax 100U, although dilution preferences may vary depending on treatment goals and injector technique.
Step 3: Inject saline slowly into the vial
Insert the needle into the Botulax 100U vial and gently inject the saline along the inner wall. Avoid shaking the vial aggressively to prevent protein denaturation.
Step 4: Allow gentle dissolution
Let the solution mix naturally until the powder is fully dissolved. The liquid should appear clear and free of particles.
Step 5: Label and store properly
Once reconstituted, label the vial with the date and time. Store it in a refrigerator at 2–8°C.
Key considerations include:
- Use sterile technique at all times
- Avoid vigorous shaking
- Do not freeze the solution
- Maintain cold chain storage
- Use within recommended time frame (typically 24 hours)
Storage conditions:
- Keep refrigerated at 2–8°C
- Do not freeze
- Avoid direct light exposure
How to Mix Botulax 200?
The preparation process for Botulax 200 is similar to that of Botulax 100, but the dosage and concentration are different.
Botulax 200U contains a higher unit volume per vial, making it more suitable for:
- Larger treatment areas
- Full-face applications
- Masseter muscle reduction
- Body contouring treatments in some protocols
Botulax 300 Dilution
The reconstitution method of Botulax 300U is the same as Botulax 100 and 200, using sterile saline under aseptic conditions. Botulax 300U is typically used for high-volume or multi-area treatments, where a higher unit concentration is required.
Botulax Dilution Chart
The dilution chart below is provided as a clinical reference to assist with accurate Botulax reconstitution and dosage preparation.
The final dilution ratio depends on treatment goals and injection technique. A higher dilution may produce a wider diffusion effect, while a lower dilution offers more precise targeting.
For clinics and professionals, mastering these fundamentals ensures consistent results, improved patient satisfaction, and higher treatment safety.
